Coding

Part:BBa_K5439001:Design

Designed by: Diego Cota Barocio, Ana Paula Valle Arce   Group: iGEM24_TecMonterreyGDL   (2024-09-28)


TjPCs (phytochelatin synthase) coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 31
    Illegal BglII site found at 1440
    Illegal XhoI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NdeI and XhoI restriction sites were added at 5' and 3' ends to clone it in pET-28b


Source

The original sequence was reported by Mizuno et al. (2003), and can be found on GenBank BAB93119.1

References

[1] Mizuno, T., Sonoda, T., Horie, K., Senoo, K., Tanaka, A., Mizuno, N., & Obata, H. (2003). Cloning and characterization of phytochelatin synthase from a Nickel hyperaccumulatorThlaspi japonicumand its expression in yeast. Soil Science & Plant Nutrition, 49(2), 285–290. https://doi.org/10.1080/00380768.2003.10410009